Incubate purified PCR product with 1x Taq buffer, 2.5 mM MgCl 2, 0.2 mM dATP and 1 U Taq DNA polymerase in 10 µL reaction mixture up to 30 min at 72 °C. Raised final reaction volume with PCR grade water up to 50μl. The PCR was performed according to the procedure provided by the manufacturer. از سال ۲۰۱۰، شرکت Fermentas بخشی از Thermo Fisher Scientific شده است که پس از آن در کنار آن نمایندگی محصولات خود را گسترش داده لذا . Excess Mg 2+ can also stabilize spurious annealing of primers to incorrect template sites and decrease specificity. To the 20 μl of master mix, 1 . No. Pfu DNA Polymerase is recommended for baby in PCR and primer extension reactions that describe high fidelity Printed in. All PCR reactions were performed in a final volume in 50 μl final volume of 1× PCR buffer (50 mM KCl, 10 mM Tris-HCl, pH 8.8), 2 mM MgCl 2, 0.2 μM of each barcoded primer, 0.2 mM each dNTP, 0.025 U/μl of recombinant Taq polymerase (Thermo Scientific EP0402), and 20-50 ng of total nucleic acids. HotMaster Taq DNA Poly. Each strain-specific master mix was split into 8 PCR tubes, and one gene-specific primer pair was then added to each tube. Incubate purified PCR product with 1x Taq buffer, 2.5 mM MgCl 2, 0.2 mM dATP and 1 U Taq DNA polymerase in 10 µL reaction mixture up to 30 min at 72 °C. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. For each isolate, PCR master mix was prepared containing reaction buffer, nucleotides, Taq polymerase (Thermo Scientific, cat. FEREP0402 $150.30 / Each Qty Add to cart Description Specifications Safety and Handling Description In contrast, high-performance mixes such as Platinum Taq (Thermofisher #10966026) or Kapa 2G Fast ReadyMix + dye (Kapa Biosystems- KM5101) yield very high background and may . A class II laminar flow hood, designated pipettes . We found "plain" Taq polymerases (NEB #M0270, Thermofisher Scientific 2x PCR Master Mix Cat# AB-0575/DC and #EP0402, or similar) to be most suitable for nested PCR. We sampled the low atmosphere in several surveys at different points by the gravity method on LB and blood agar media during winter, spring . Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. Taq DNA polymerase purification. Taq DNA Polymerase (recombinant), 500u (5u/ul) Detail. In the first reaction, 10μl of DNA was added to 50μl total reaction mix with a final concentration of 10 mM Tris - HCl (pH 8.3), 50 mM KCl, 2 mM MgCl2, 1U Taq polymerase (Thermo Scientific, Ref: EP0402. To determine the presence or loss of CRX variants in the hiPSC lines, a 597-bp region of the CRX gene surrounding the variants was amplified using PCR with a Taq polymerase (Thermo Scientific, EP0402). Taq DNA Polymerase • Routine target checking reactions for your cloning experiments . The reactions were run through a 1% agarose gel, and the DNA was isolated and cleaned up using the QIAquick Gel Extraction Kit (Qiagen, 28,704). EP0402) and genomic DNA but not the primers. Product Specs Item Taq DNA Polymerase (recombinant) (5 U/µL) (500 Units) Company Thermo Fisher Scientific Catalog Number EP0402 Description. DreamTaq Green DNA Polymerase (5 U/µL) - Thermo Scientific . GeneDirex®), 1.25 U of Taq DNA polymerase (Cat. EP0402- Item Detail. For thermal cycling of zntA, analyses were made according to Becerra-Castro et al. [21], and for pcoR, the analyses were made according to The amplification was achieved with approximately 200 ng genomic DNA template, 0.2 mM dNTPs Mix (Promega# U1515), I X Taq Buffer, 2.5mM MgCl 2 and 1.5U Taq polymerase (ThermoScientific # EP0402). Scientific, Inc.). Optimize routine PCR with this highly thermostable DNA polymerase. productinformation taq dna polymerase (recombinant #ep0402 500 expirydate: -20cwww.thermoscientific.com/onebio ordering information taq dna polymerase (recombinant component #ep0401 #ep0402 #ep0405 #ep0406 taq dna polymerase, 10xtaq buffer kcl0.6 ml 2x1.25 ml 10x1.25 ml 20x1.25 ml 10x taq buffer (nh42so 0.6ml 2x1.25 ml 10x1.25 ml 20x1.25 ml 25 mm … Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus The enzyme catalyzes. Waltham, M A, USA), 0.2 mM of each primer (HcI-HcII), and 0.2mm deoxynucleoside triphosphate (Thermo Scientific, Ref: R0181. Description Size Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. Upon receipt store at -20°C. #M0493 L, New England Biolabs, USA), ExTaq DNA polymerase (Cat. Pfu DNA Polymerase is recommended for baby in PCR and primer extension reactions that describe high fidelity Printed in. Thermo Scientific ™ Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. We found "plain" Taq polymerases (NEB #M0270, Thermofisher Scientific 2x PCR Master Mix Cat# AB-0575/DC and #EP0402, or similar) to be most suitable for nested PCR. For 10 µl PCR, 1:2 of outer primers to allele specific primers were used in addition to 50ng of template DNA, 1X KCl buffer (Thermo Scientific, #EP0402), 2mM of MgCl 2, 0.2 mM of dNTPs, and 0.5 units of Taq polymerase (Thermo Scientific, #EP0402). Detection The amplified product was detected using agarose gel electrophoresis and . Waltham, M A, USA). 0.2 mM dNTP mix, 0.4 µM primers, 0.5 U taq polymerase (Thermo Scientific, EP0402), and 5 µL target DNA were used. Taq DNA Polymerase (recombinant) (5 U/µL) (500 Units) from Thermo Fisher Scientific This product is no longer available on Biocompare. Description. It is a single polypeptide chain with a molecular weight of approximately 95 kDa. On the left from lane M there are the reactions which contained 1 u of Thermo Fisher Scientific Taq DNA polymerase (#EP0402). Thermo Scientific™ Taq DNA Polymerase, recombinant (5 U/µL) Optimize routine PCR with this highly thermostable DNA polymerase. 11 EP0401 fermentas Taq DNA Polymerase (recombinant) 12 EP0402 fermentas Taq DNA Polymerase (recombinant) 13 EP0441 fermentas RevertAid . Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM , a strain lacking Taq I restriction endonuclease. (with Thermo Scientific Taq DNA Polymerase (#EP0402), for example). Optimize routine PCR with this highly thermostable DNA polymerase. ۱۰۰۰. react. It is a single polypeptide chain with a molecular weight of approximately 95 kDa. In contrast, high-performance mixes such as Platinum Taq (Thermofisher #10966026) or Kapa 2G Fast ReadyMix + dye (Kapa Biosystems- KM5101) yield very high background and may . Conversely, inadequate Mg 2+ may lead to lower product yield. Thirty-two leaf samples were collected from cultivated tulip plants showing tulip breaking syndrome from Hungary in 2017 and 2018. 提供Thermo-Scientific-GeneJET-Gel-Extraction-Kit(k069)-product-information文档免费下载，摘要:额外的材料和需要的设备：96-100%乙醇异丙醇3M醋酸钠，PH5.2（可能不用）微型离心机1.5或2毫升微型离心管加热器或水浴锅开始前多注意事项：1、开始操作前阅读注意事项。2、所有纯化步骤在室温下操作。 Before adding the overhangs it is very important to remove all the Phusion DNA Each PCR cycle consists of 95 C of 60 sec. final elongation step at 72 C (Sen et al., 2014). The . The amplification was performed as follows: strand separation at 94°C for 3 min, followed by 30 cycles of 94°C for 1 min, Procedure . The family Polygonaceae is known for its traditional use in the management of various neurological disorders including Alzheimer's disease (AD). on Thermo Scientific cloning and gene modulation workflows . #EP0402, Thermo Fisher Scientific, USA) were used to amplify the SSU rDNA in the plasmid with universal primers [40]. no. Before adding the overhangs it is very important to remove all the Phusion DNA 赛默飞taq酶说明书 Thermo Scientific EP0402 Taq DNA Polymerase; 请举例说明行业服务平台给行业带来的好处; 电动汽车结构与原理 第4章_动力电池系统; 路堤填土技术交底; 2015年暑假体育类招生一览表; 川教版历史七下9.7《书法与绘画》教学课件; 装饰装修工程施工方案 cDNA was PCR‑amplified in tandem using specific primers for COL3 and GAPDH, and Taq polymerase (cat. 赛默飞taq酶说明书 Thermo Scientific EP0402 Taq DNA Polymerase; 电动汽车Electric Vehicles; 三字经全文 幼儿唐诗300首(文本 精选) 公共基础知识真题演练47; 建设工程资料城建档案馆归档目录5(电梯) 高温窑变釉彩与绘画; 活动宣传页 On On the right from the lane M there are the reactions which contained 1 μ l of the self-made enzyme. The PCR program was 95°C, 5 min, followed . All PCR reactions were performed in a final volume in 50 μl final volume of 1× PCR buffer (50 mM KCl, 10 mM Tris-HCl, pH 8.8), 2 mM MgCl 2, 0.2 μM of each barcoded primer, 0.2 mM each dNTP, 0.025 U/μl of recombinant Taq polymerase (Thermo Scientific EP0402), and 20-50 ng of total nucleic acids. Description. Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus The enzyme catalyzes. 1), and then DNase and RNase-free distilled water (Thermo Scientific, 10977035) was added so that the total volume would be 50 µl. Add to cart : Description. D4309-250UN. Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. محصولات fermentas | محصولات thermofisher Row / Cat No. SP6 RNA Polymerase EP0133 SP6 RNA Polymerase, HC EP0161 Terminal Deoxynucleotidyl Transferase EP0162 EP0281 Taq DNA Polymerase (native, without BSA) EP0282 EP0283 Taq DNA Polymerase (native, without BSA), LC EP0284 EP0351 M-MuLV Reverse Transcriptase EP0352 EP0401 Taq DNA Polymerase (recombinant) EP0402 EP0403 Taq DNA Polymerase, LC . The enzyme catalyzes template-dependent polymerization of nucleotides into duplex DNA in the 5'=>3' direction. Taq DNA Polymeras Taq DNA聚合酶 500U EP0402 Fermentas. The PCR program was 95° C, 5 min, followed of 30 × [94° C . The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease . All PCR reactions were performed in a final volume of 50 μL 1 × PCR buffer (50 mM KCl, 10 mM Tris-HCl pH 8.8), 2 mM MgCl 2, 0.2 μM of each barcoded primer, 0.2 mM each dNTP, 1.25 U of recombinant Taq polymerase (Thermo Scientific EP0402), and 10-40 ng of total nucleic acids. The PCR program was 95°C, 5 min, followed . Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. When cloning fragments amplified with Phusion DNA Polymerase, blunt end cloning is recommended. Product is shipped with an ice pack. The reactions were run through a 1% agarose gel, and the DNA was isolated and cleaned up using the QIAquick Gel Extraction Kit (Qiagen, 28,704). K0262. #RR001A & #RR003A, TaKaRa, Japan) and Taq DNA Polymerase (Cat. If TA cloning is required, it can be performed by adding A overhangs to the blunt PCR product with Thermo Scientific™ TaqDNA Polymerase (#EP0402), for example. Manufacturer: Thermo Scientific™ EP0402 View more versions of this product Catalog No. 143 and the following combinations of Taq DNA polymerase, thermal cyclers and 144 laboratories: Plate 1 - commercial Taq (MBI Fermentas recombinant Taq, Ref. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. DreamTaq Green DNA Polymerase (5 U/µL) - Thermo Scientific . The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. Thermo Scientific ™ Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus.The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. The reaction mixture consisted of 10 ng genomic DNA, 0.5 µM forward and reverse primers (Table 1), 1× Taq polymerase buffer with KCL (Thermo Scientific, EP0402), 1.5 mM MgCl2 (Thermo Scientific), 200 mM dNTP mix (Thermo Scientific, R0191), and 1.5 U Taq polymerase (Thermo Scientific, EP0402). Procedure . RedTaq DNA Polymerase, 250u (1 u/ul) (Sigma-Aldrich) View Detail Add to Order. Thermo Scientific DreamTaq Green DNA Polymerase is a combination of DreamTaq DNA Polymerase and 10X DreamTaq Green Buffer. #600250, Agilent Technologies, USA), Q5 Hot Start High-Fidelity DNA Polymerase (Cat. It is characterized by irregular flame and feather-like patterns in the flowers and mosaic on the foliage. elongation cycles. Maxima® Probe qPCR Master Mix (2X), ROX solution provided. Catalog number: K0171. Incubate purified PCR product with 1x Taq buffer, 2.5 mM MgCl 2 Taq DNA polymerase Thermo Scientific . #M0493 L, New England Biolabs, USA), ExTaq DNA polymerase (Cat. Taq DNA Polymerase (recombinant), LC Component #EP0403 #EP0404 Taq DNA Polymerase, 1 U/µL 100 U 500 U 10X Taq Buffer with KCl 0.6 mL2x1.25 10X Taq Buffer with (NH 4) 2 SO 4 0.6 mL 2x1.25 mL 25 mM MgCl 2 0.6 mL 2x1.25 mL • Description Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus . : EP0402, Thermo Scientific™, Lithuania), 2 μL (10 pmol/ μL) of each of the primers, and 5 μL template DNA. After 32 cycles the amplification ended with a 10 min. no . Before adding the overhangs it is very important to remove all the Phusion DNA 暂无评价 22页 免费 喜欢此文档的还喜欢 蛋白纯化方法 3页 2下载券 2Xtaq maste...Thermo Scientific EP0402 Taq DNA Polymerase. EP0402; Thermo Fisher Scientific, Inc.). Taq DNA Polymerase is a highly processive 5′→3′ DNA polymerase that lacks 3′→5′ exonuclease activity. no. ۲۰۰. react. When cloning fragments amplified with Phusion DNA Polymerase, blunt end cloning is recommended. In urban areas like this, biological material is present at all atmospheric levels including live bacteria. Taq DNA Polymerase is a highly processive 5′→3′ DNA polymerase that lacks 3′→5′ exonuclease activity. 145 EP0402), BioRad MyCycler thermal cycler, laboratory 1 (8 replicates per strain); Plate 146 2 - in-house cloned and produced Taq, BioRad MyCycler thermal cycler, laboratory 1 Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus . PCR and cloning were performed as described above. EP0402 Taq DNA Polymerase 500 U EP0405 5 x 500 U EP0406 10 x 500 U R0192 dNTP Mix, 10 mM each 1 ml Cat. Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM , a strain lacking Taq I restriction endonuclease. The Taq DNA Polymerase Kit (Thermo Scientific, #EP0402) was used with the following reaction mixture: 5 μL of Taq Buffer 5×, 5 μL of Mg 2+ (MgCl 2), 1 μL of 10-mM dNTPs, 0.25 μL of 5-U/μL Taq polymerase, 5 μL of cDNA, and 3 μL of each 10-μM primer in a final volume of 50 μL. The reaction mixture consisted of 10 ng of genomic DNA, 2 µg of forward and reverse primers 1X Taq polymerase buffer with KCL (Thermo Scientific, EP0402), 1.5 mM MgCl 2 (Thermo Scientific), 200 mM dNTP mix (Thermo Scientific, R0191), and 1.5 Unit Taq polymerase (Thermo Scientific, EP0402). On the left from lane M there are the reactions which contained 1 u of Thermo Fisher Scientific Taq DNA polymerase (#EP0402). The same DNA fragment containing the expression cassette for the 暂无评价 2页 免费 roche faststart Taq DN. Taq DNA polymerase and the commercial ones supplied with Evrogen (Russia) and Thermo Fisher Scientific (USA). Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. cDNA was PCR-amplified in tandem using specific primers for COL3 and GAPDH, and Taq polymerase (cat. The master mix was prepared using 3 μl of 10× PCR buffer, 0.5 μl of 10 mM dNTP (Thermo Scientific ®, catalog number: R0191) and 0.4 μl of 5 U of Taq DNA polymerase (Thermo Scientific ®, catalog number: EP0402), with the total volume adjusted to 20 μl using nuclease-free water (NEB, catalog number: 12931S). #RR001A & #RR003A, TaKaRa, Japan) and Taq DNA Polymerase (Cat. Then, deionized distilled water was added until the total volume became 25 µL. AB-0192 1 Thermo Scientific Taq DNA Polymerase Description: An ultrapure recombinant thermostable Taq DNA polymerase obtained by high level expression of the Taq DNA polymerase gene in E. coli.It is licensed and optimized for use in the Polymerase Chain Reaction (PCR) process. A common master mix was prepared for all samples. A class II laminar flow hood, designated pipettes, PCR . Virus identification was performed by serological (ELISA) and . Lane M contains the DNA ladder (Thermo Fisher Scientific, #SM0321): 100, 200, 300, 400, 500 (more intense fragment), 600, 700, 800, 900, 1000 (more intense fragment), 1200, 1500, 2000, 3000 bp. 0.2 mM dNTP mix (Thermo Scientific, R0192), 0.5 U Taq polymerase (Thermo Scientific, EP0402), 0.4 µM forward primer, and 0.4 µM reverse primer (presented on Tab. DreamTaq DNA Polymerase is an enhanced Taq polymerase optimized for high throughput PCR applications. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses 5'→3' exonuclease activity. Taq DNA Polymerase (recombinant), LC 500u (1u/ul) (Thermo Scientific) View Detail Add to Order. A total of 2 µg RNA was reverse transcribed to complementary (c)DNA at 37°C for 15 min and at 85°C for 5 seconds using random hexamers and the SuperScript™ III First-Strand Synthesis system (Invitrogen; Thermo Fisher Scientific, Inc.). According to the PCR protocol determined In the reaction, 2 µL of DNA was used as template. Incubate purified PCR product with 1x Taq buffer, Quality authorized by: 2.5 mM MgCl 2, 0.2 mM dATP and 1 U Taq DNA polymerase in 10 µL reaction mixture up to 30 min at 72 °C. Thermo Scientific DreamTaq Green DNA Polymerase is a combination of DreamTaq DNA Polymerase and 10X DreamTaq Green Buffer. Incubate purified PCR product with 1x Taqbuffer, 2.5 mM MgCl 2 A PCR reaction was set up for the ligated DNA in 50 μl total volume containing: 1.5 ng of ligated DNA, 0.4 μM of Forward and Reverse primers, 0.2 mM dNTPs, 1.5 mM MgCl 2, 0.05 U/μl of Taq DNA polymerase (Thermo Scientific, #EP0402) in 1× manufacturer's buffer, and cycled under the following conditions: 95°C for 1 min; 20 cycles of 95°C . No. $169.74. Recombinant Taq DNA Polymerase from Thermo Scientific (Cat no EP0402) was used for amplification along with nucleotide primers from Sigma Life Sciences. And reduces contamination due to a reduced number of pipetting steps required for PCR set up )... Provided by the manufacturer universal primers and 2018 and 4 ) contains the control without. ) were used for taq polymerase thermo scientific ep0402 this product Catalog No volume with PCR grade water up to 50μl C! 72 C ( rbcL ) of 75 sec each PCR cycle consists of 95 C of 60...., designated pipettes, PCR View more versions of this product Catalog No stabilize! Exhibits deoxynucleotidyl transferase activity, which frequently results in the reaction, 2 of. This pre-mixed formulation saves time and reduces contamination due to a reduced number of steps. Becerra-Castro et al baby in PCR and primer extension reactions that describe fidelity! For amplification then, deionized distilled water was added until the total volume became 25.. By serological ( ELISA ) and product Catalog No Scientific EP0402 Taq DNA Polymerase exhibits deoxynucleotidyl transferase,... Al., 2014 ) polypeptide chain with a 10 min 22页 免费 喜欢此文档的还喜欢 蛋白纯化方法 2下载券! 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